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KMID : 0861020180330040035
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Korea Journal of Herbology
2018 Volume.33 No. 4 p.35 ~ p.41
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Investigation of Antimicrobial and Anti-inflammatory Activities of the Hyeonggaeyeongyotang Gagambang
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Gang Seong-Gu
Cho Nam-Joon
Kim Ji-Young
Han Hyo-Sang
Kim Kee-Kwang
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Abstract
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Objectives : Hyeonggaeyeongyotang Gagambang (HYT) is a herbal medicine prescribed for the treatment of inflammatory diseases, but it is necessary to study the exact therapeutic efficacy. This study aims to investigate the antibacterial and anti-inflmmatory activities of HYT.
Methods : Antibacterial activity of HYT was confirmed by staining Escherichia coli , a gram negative strain, and Staphylococcus aureus , a gram positive strain, on solid Lysogeny Broth (LB) medium containing HYT. Antioxidant activity of HYT was confirmed by 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay. The phosphorylation of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) after lipopolysaccharide (LPS) treatment with HYT-treated RAW 264.7 mouse macrophages cells was confirmed by immunoblot analysis and the level of interleukin 1 beta (IL-1β) mRNA expression level was confirmed by quantitative real-time PCR.
Results : HYT showed a concentration-dependent antibacterial activity against Escherichia coli and Staphylococcus aureus and also showed excellent antioxidant activity. HYT treatment attenuated the phosphorylation of IκBα induced by LPS treatment in RAW 264.7 mouse macrophages cells. The phosphorylation of IκBα is crucial for the regulation of the expression of various pro-inflammatory cytokines. In addition, IL-1β mRNA expression level of RAW 264.7 mouse macrophages cells stimulated by LPS treatment was also inhibited by HYT treatment.
Conclusions : Through experimental demonstration of the antioxidative, antimicrobial and anti-inflammatory effects of HYT, we demonstrated that HYT is a herbal medicine effective for the treatment of inflammatory diseases caused by various bacterial infections.
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KEYWORD
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Hyeonggaeyeongyotang Gagambang, Antimicrobial effect, Antioxidant effect, Anti-inflammatory effect, RAW 264.7 cell
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